Phosphorylation of choline and ethanolamine in Ehrlich ascites-carcinoma cells

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Great Britain Phosphorylation of Choline and Ethanolamine in Ehrlich Ascites - Carcinoma Cells BY CHENG - PO

1. Ehrlich ascites-cell extracts convert choline and ethanolamine approximately equally well into their respective phosphoryl derivatives. 2. Choline is a potent inhibitor of ethanolamine phosphorylation, but ethanolamine has little effect on choline phosphorylation. 3. 2,3-Dimercaptopropanol, cysteine and Ca2+ inhibit ethanolamine phosphorylation, but have no detectable effect on choline phosp...

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Effects of Ehrlich ascites antisera on the biochemical activities of Ehrlich ascites carcinoma cells in vitro.

Anl i-tumor sera are now being extensively used in investigations of the activities of neoplasms (e.g. 4-7, 9-13, 15). I t is already known (7), concerning their effects on the biochemical activities of tumors, that Ehrlich ascites carcinoma cells, treated with their antisera in the presence of comp]ement, lose their capacity to respire in the presence of glucose but not in the presence of succ...

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Transport and metabolism of thiamine in Ehrlich ascites-carcinoma cells.

1. Aerobic or anaerobic incubation at 37 degrees of Ehrlich ascites-carcinoma cells in Krebs-Ringer bicarbonate medium containing glucose and labelled thiamine results in accumulation in the cell of labelled thiamine, so that the concentration of total labelled thiamine in the cells greatly exceeds (by a factor 7) that in the medium. This concentration ratio is approximately constant for small ...

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Inorganic ion concentrations in Ehrlich ascites carcinoma cells and fluid.

The concentrations of sodium, potassium, magnesium, cal cium, chloride, and phosphate were determined in Ehrlich ascites tumor cells and fluid after 5, 7, 9, and 12 days of growth. Material obtained after the 12th day showed peculiar intra cellular ion patterns, presumably related to the near-terminal condition. Material drawn after 5-9 days of growth represented viable cells. The intracellular...

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Amethopterin transport in Ehrlich ascites carcinoma and L1210 cells.

Accumulation of amethopterin by L1210 and Ehrlich ascites cells in miro was mediated by a slow, temperature-sensitive, and partly saturable process. The structural specificity of the proc ess could be measured by competition studies; differences were found between the two cell lines. At physiologic drug levels, i.e., those found in vivo during drug therapy, concentrativi uptake of amethopterin ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1967

ISSN: 0306-3283

DOI: 10.1042/bj1050497